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Biogate experts will make every effort to provide customers with the development and production of antigens and antibodies in the shortest possible time and at a competitive price. Contact us with your custom antibody development project for a quote.
Our scientists can provide immediate answers to common questions and will get your project started within several days. We will keep you informed of your project's status and notify you when materials are being shipped to your laboratories. |
Monoclonal Antibody Development |
Mouse hybridoma technology is used to produce monoclonal antibodies. A period of three to nine months will normally be required for completion of the project. The general steps and our methods are discussed below:
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Phase 1: Immunization
Phase 2: Cell Fusion and Screening Phase 3: Cloning Phase 4: Pilot Production Phase 5: Antibody Purification |
With the client-specified antigen, mice will be immunized with either a client-specified method or our own recommended method. Mice sera will be checked periodically for specific antibodies by such assay systems as ELISA, Western Blotting, or a specified assay. Sera can also be made available to clients for further examination before our proceeding with cell fusion.
Spleen cells are fused with myeloma cells. The fused cells are then placed in several 96-well culture plates. Viable colonies appear as early as 3-5 days after fusion and are kept healthy with a fresh supply of nutrient media as necessary. When hybridoma colonies are large enough to screen, screening of all wells containing fusion products will be done with ELISA assay. All positive wells will be expanded and frozen for parental hybridomas. The number of positives depends on the nature of the antigen. Cloning dilution is performed on selected clones to ensure activity and monoclonality of the specific antibody. Once antibody activity and monoclonality is ensured, cells will be injected into mice for ascites production. Ascites will then be collected and frozen. The best method of purification will be used to ensure the best combination of highest purity and activity possible. A report of the proposed purification strategies and any relevant issues that might arise will be made available to the client and performed upon approval. |
Polyclonal Antibody Production
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Biogate offers custom polyclonal antibody production for a client's specific applications. A typical project requires at least three months to complete. We can accommodate small, short-term research projects as well as long-term production programs at any scale, in compliance with Good Manufacturing Practice (GMP), using goats, rabbits, or chickens.
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