S. Typhoid IgG/IgM
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Typhoid fever is a life threatening illness caused by the bacterium Salmonella typhus, and was observed by Eberth (1880) in the mesenteric nodes and spleen of fatal cases of typhoid fever. It is common in developing countries where it affects about 12.5 million persons annually. The infection is acquired typically by ingestion. On reaching the gut, the bacilli attach themselves to the epithelial cells of the intestinal villi and penetrate the lamina and submucosa. They are then phagocytosed there by polymorphs and mesenteric lymph nodes, where they multiply and, via the thoracic duct, enter the blood stream. A transient bacteremia follows, during which the bacilli are seeded in the liver, gall bladder, spleen, bone marrow, lymph nodes, and kidneys, where further multiplication takes place. Towards the end of the incubation period, there occurs a massive bacteremia from these sites, heralding the onset of the clinical symptoms. The diagnosis of typhoid consists of isolation of the bacilli and the demonstration of antibodies. The isolation of the bacilli is very time consuming and antibody detection is not very specific. Other tests include the Widal reaction.
The Salmonella typhi IgG/IgM Test Device (Serum/Plasma/Whole Blood) is a qualitative test for the detection of IgG and IgM antibodies to S. typhi in human serum, plasma, or whole blood. The test provides a differential detection of anti-S. typhi-IgG and anti-S. typhi -IgM antibodies and can be used for the presumptive distinction between a current, latent and/or carrier S. typhi infection. Serum, plasma or whole blood samples may be used with this test. First a specimen is dispensed into the sample well of the test device. If IgG or IgM antibodies to S. typhi are present in the specimen they will bind to the colloidal gold-antigen conjugate and travel up the membrane chromatographically. The antibody-Antigen-colloidal gold complex will then bind to the immobilized anti-Human IgG and/or anti-Human IgM coated on the membrane. This will cause pale to dark colored lines to form at the IgG or IgM test region and can be seen in the results window. The intensity of the lines will vary depending upon the amount of antibody present in the sample. The appearance of a colored line in a specific test region should be considered as positive for that particular antibody (IgG and/or IgM). To serve as a procedural control, a colored line will always appear in the control line region indicating that proper volume of specimen has been added and proper membrane wicking has occurred.
The Salmonella typhi IgG/IgM Test Device (Serum/Plasma/Whole Blood) is a qualitative test for the detection of IgG and IgM antibodies to S. typhi in human serum, plasma, or whole blood. The test provides a differential detection of anti-S. typhi-IgG and anti-S. typhi -IgM antibodies and can be used for the presumptive distinction between a current, latent and/or carrier S. typhi infection. Serum, plasma or whole blood samples may be used with this test. First a specimen is dispensed into the sample well of the test device. If IgG or IgM antibodies to S. typhi are present in the specimen they will bind to the colloidal gold-antigen conjugate and travel up the membrane chromatographically. The antibody-Antigen-colloidal gold complex will then bind to the immobilized anti-Human IgG and/or anti-Human IgM coated on the membrane. This will cause pale to dark colored lines to form at the IgG or IgM test region and can be seen in the results window. The intensity of the lines will vary depending upon the amount of antibody present in the sample. The appearance of a colored line in a specific test region should be considered as positive for that particular antibody (IgG and/or IgM). To serve as a procedural control, a colored line will always appear in the control line region indicating that proper volume of specimen has been added and proper membrane wicking has occurred.
CatalogRST-016C
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ProductS. Typhoid IgG/IgM
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FormatCassette
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Specimen *WB/S/P
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Kit Size20 test
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* whole blood test could be conducted with assistant of assay buffer; but it may not work for every patient due to blood coagulation
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or sales@biogatelab.com for more information sample, order, product information and business cooperation.
or sales@biogatelab.com for more information sample, order, product information and business cooperation.