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Hepatitis C virus (HCV) now is recognized as a major agent of chronic hepatitis, transfusionacquired non-A, non-B hepatitis and liver disease throughout the world. HCV is an enveloped positive-sense, single-stranded RNA virus. Clinical diagnostic issues related to HCV is the detection of HCV antibodies in human serum, plasma or whole blood by immunoassay. We have constructed HCV genes for the expression of recombinant antigens in bacterium systems such as E. coli and focused on structural and non-structural regions of HCV-encoded polyprotein, which are definitely immunogenic. The major immunoreactive antigens of these proteins have been reported as core, NS3, NS4 and NS5 regions of HCV genome, which are known to be highly immunodominant regions. For diagnosis of HCV infection, these recombinant proteins were used as capture materials of a immunochromatographic (rapid) test. Compared to the first generation HCV test using single recombinant antigens, multiple antigens using recombinant proteins have been added in new serologic tests to avoid nonspecific cross-activity and to increase the sensitivity of the HCV antibody test.
The One Step HCV Test is a qualitative membrane strip based immunoassay for the detection of HCV antibodies in Whole Blood /Serum / Plasma. In this test procedure, recombinant HCV antigen is immobilized in the test line region of the device. After a Whole Blood /Serum / Plasma specimen is placed in the specimen well, it reacts with HCV antigen coated particles that have been applied to the specimen pad. This mixture migrates chromatographically along the length of the test strip and interacts with the immobilized HCV antigen. If the specimen contains HCV antibodies, a colored line will appear in the test line region indicating a positive result. If the specimen does not contain HCV antibodies, a colored line will not appear in this region indicating a negative result. To serve as a procedural control, a colored line will always appear at the control line region indicating that proper volume of specimen has been added and membrane wicking has occurred.
The One Step HCV Test is a qualitative membrane strip based immunoassay for the detection of HCV antibodies in Whole Blood /Serum / Plasma. In this test procedure, recombinant HCV antigen is immobilized in the test line region of the device. After a Whole Blood /Serum / Plasma specimen is placed in the specimen well, it reacts with HCV antigen coated particles that have been applied to the specimen pad. This mixture migrates chromatographically along the length of the test strip and interacts with the immobilized HCV antigen. If the specimen contains HCV antibodies, a colored line will appear in the test line region indicating a positive result. If the specimen does not contain HCV antibodies, a colored line will not appear in this region indicating a negative result. To serve as a procedural control, a colored line will always appear at the control line region indicating that proper volume of specimen has been added and membrane wicking has occurred.
CatalogRHE-049C
RHE-049C |
ProductHCV
HCV |
FormatStrip
Cassette |
Specimen *S/P
S/P |
Kit Size50 test
25 test |
* whole blood test could be conducted with assistant of assay buffer; but it may not work for every patient due to blood coagulation
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Please contact sales@biogatelab.ca
or sales@biogatelab.com for more information about sample, order, product information and business cooperation.
or sales@biogatelab.com for more information about sample, order, product information and business cooperation.